If you need bacteriostatic water alongside lyophilised peptides for a full reconstitution kit, our Peptide Healing Stack (BPC-157 5 mg + TB-500 5 mg + BAC water) bundles both peptides with the reconstitution water in one parcel — useful for users running combined recovery protocols.
Quick Answer — What is Bacteriostatic Water?
Bacteriostatic (BAC) water is sterile, non-pyrogenic water containing 0.9% benzyl alcohol as a preservative. The benzyl alcohol inhibits microbial growth, which means the same multi-use vial can be accessed repeatedly under proper sterile technique — unlike plain sterile water, which must be discarded after a single puncture. It is the standard diluent for reconstituting lyophilized research peptides such as BPC-157, TB-500, GHK-Cu, Semax, and Adamax.
BAC Water — Sterile Multi-Dose Diluent for Research Peptides
Bacteriostatic Water for Injection is the single most common reconstitution fluid used in peptide research. It is pharmaceutical-grade sterile water to which 0.9% benzyl alcohol has been added as a bacteriostatic preservative. That small but critical addition is what allows a single 10 mL vial to be punctured and drawn from multiple times over a 28-day period without microbial contamination — a practical advantage in any laboratory protocol that requires repeated dosing from lyophilized peptide stock.
Every peptide sold on MedsBase arrives freeze-dried (lyophilized). Before use, the powder must be dissolved in a compatible liquid to produce a solution that can be measured by volume. BAC Water is the industry-standard choice because it delivers three properties simultaneously: sterility, pyrogen-free status, and multi-dose compatibility.
Why Bacteriostatic Water Rather Than Sterile Water?
Sterile Water for Injection is plain water that has been filtered and autoclaved. Once the rubber stopper of a sterile-water vial is punctured by a needle, the contents should be used immediately and the vial discarded, because no preservative is present to suppress bacterial or fungal growth introduced by that puncture.
Bacteriostatic Water adds 0.9% benzyl alcohol — a concentration well below any cytotoxic threshold but high enough to keep common environmental organisms (Staphylococcus epidermidis, Escherichia coli, Candida albicans) from proliferating in the residual liquid between draws. Research workflows that reconstitute a 5 mg peptide vial into 2 mL and then dose 200 µg increments every 24 hours over 10–25 days depend on this multi-dose capability.
Reconstitution Protocol — Step by Step
The protocol below is the standard method used in peptide research laboratories. For a more detailed treatment of dilution math, concentration calculations, and troubleshooting, see our BAC Water reconstitutiegids.
- Prepare the workspace. Wipe down a clean flat surface with 70% isopropyl alcohol. Wash hands and put on disposable gloves.
- Inspect the vials. BAC Water should be clear and colorless. The lyophilized peptide should appear as a white or off-white powder or cake; any discoloration or visible particles means the vial should not be used.
- Wipe both rubber stoppers with alcohol. Let them dry for 15–20 seconds — needle puncture through a still-wet stopper can push alcohol into the vial.
- Draw up BAC Water into a syringe. The standard working dilution for most peptides is 2 mL of BAC Water per 5 mg of peptide, giving a final concentration of 2.5 mg/mL (or 250 µg per 0.1 mL mark on an insulin syringe).
- Inject the BAC Water gently down the side of the peptide vial. Do not squirt it directly onto the lyophilized powder — that can cause foaming and local denaturation. Let the liquid run down the inner glass wall.
- Swirl gently — do not shake. Most peptides dissolve within 30–60 seconds of gentle swirling. Vigorous shaking generates air-water interface turbulence that can fragment peptide structures, particularly larger compounds like TB-500 and Thymosin Alpha-1.
- Label the reconstituted vial. Write the reconstitution date on the vial. Refrigerated storage and use within 30 days is standard; beyond that, degradation products may accumulate.
Compatibility With Research Peptides
BAC Water is compatible with essentially every research peptide sold on MedsBase, including:
- BPC-157 — tissue repair research peptide; reconstitutes cleanly at 2.5–5 mg/mL.
- TB-500 (Thymosin Beta-4) — systemic recovery peptide; avoid vigorous shaking during reconstitution.
- GHK-Cu (Copper Peptide) — the copper chelation is stable in BAC Water at typical working concentrations.
- Semax en Adamax — short nootropic peptides with good BAC Water solubility.
- Growth-hormone-releasing peptides (Ipamorelin, CJC-1295, Sermorelin) — reconstitute at 1–2 mg/mL for typical research dosing.
A small number of research compounds are supplied with a specific recommended diluent (sterile water or acetic acid solution). Always check product-specific documentation before defaulting to BAC Water.
Opslag
- Unopened vials: store at controlled room temperature (15–25 °C) protected from direct light. Refrigeration is not required.
- Once punctured: standard laboratory practice is to discard after 28 days even if solution remains clear. The 0.9% benzyl alcohol concentration is designed for this service life.
- Reconstituted peptide solutions: refrigerate at 2–8 °C and use within 30 days for most peptides (shorter for larger or less stable compounds).
Safety Considerations
The 0.9% benzyl alcohol content has two documented safety considerations in clinical research:
- Neonatal research protocols avoid BAC Water entirely. Large-volume benzyl alcohol exposure in neonates has been associated with “gasping syndrome.” Research involving neonatal tissue or subjects should use plain sterile water instead.
- Benzyl alcohol hypersensitivity. Rare but documented. Any research protocol reporting unexpected injection-site reactions should rule out benzyl alcohol sensitivity before attributing the response to the peptide itself.
For the overwhelming majority of peptide research applications, the 0.9% concentration is well-tolerated and the multi-dose advantage outweighs any concern.
Why order BAC Water from MedsBase
- Onderzoekskwaliteit voorraad — bacteriostatic water supplied as lyophilized powder at HPLC ≥99% purity, with certificate of analysis available on request.
- Discrete wereldwijde verzending — eenvoudige temperatuurstabiele verpakking, wereldwijde verzending via onze speciale peptidekoerier zonder extra kosten bij peptide-only bestellingen.
- Transparante prijzen — getrapte kitkortingen op multi-vial packs; single-vial bestellingen beschikbaar voor kleine protocollen.
- Koudeketenbehandeling — temperatuurgecontroleerde verzending voor peptidezendingen om de integriteit van de verbinding tijdens het transport te beschermen.
- Geverifieerde klantbeoordelingen — lees wat 1.400+ klanten uit 50+ landen hebben gerapporteerd op onze reviews pagina.
📦 Elke bestelling is gedekt door onze Reshipment Assurance Policy — als uw pakket niet binnen 20 werkdagen arriveert, sturen wij het opnieuw.
| Specificatie | Detail |
|---|---|
| CAS-nummer | Sterile water (CAS 7732-18-5) + 0.9% benzyl alcohol (CAS 100-51-6) |
| Molecuulformule | H2O + C6H5CH2OH (0.9%) |
| Moleculair gewicht | n/a (multi-component diluent) |
| Sequentie | n/a (sterile multi-dose injection diluent — not a peptide) |
| Form | Lyofiliseerd poeder (of zoals geleverd) |
| Zuiverheid | ≥99% (HPLC geverifieerd, COA op aanvraag) |
| Opslag | 15–30 °C (controlled room temperature). Protect from light. Use opened multi-dose vial within 28 days of first puncture. |
| Oplosbaarheid | Bacteriostatisch water (aanbevolen) of steriel water voor kortere gebruiksperioden |
| Onderzoeksgebruik | Alleen voor laboratoriumonderzoek. Niet voor humaan of veterinair diagnostisch of therapeutisch gebruik. |
Veelgestelde vragen
Can I reuse a BAC Water vial for multiple peptides?
Yes — that is the primary advantage of bacteriostatic water over sterile water. A single 10 mL BAC Water vial can be drawn from repeatedly for up to 28 days under proper sterile technique (wipe the stopper with 70% IPA before every puncture, use a new sterile needle each time).
How long does BAC Water last once opened?
Standard laboratory practice is 28 days from first puncture, stored at room temperature. The 0.9% benzyl alcohol concentration is specifically designed for this in-use period.
What is the difference between BAC Water and sterile water?
Sterile water contains no preservative and must be used within a single session once punctured. Bacteriostatic water contains 0.9% benzyl alcohol, allowing safe multi-dose access for up to 28 days. For peptide research that requires sequential dosing from a reconstituted stock, BAC Water is almost always the correct choice.
What is the correct dilution ratio for BPC-157 or TB-500?
The most common working dilution is 2 mL of BAC Water per 5 mg of peptide, giving 2.5 mg/mL. On an insulin syringe marked in 0.01 mL increments, that means 250 µg per 0.1 mL (10-unit mark). Adjust based on your protocol’s target dose per injection.
Can BAC Water be used to reconstitute HCG?
Yes — BAC Water is the standard diluent for reconstituting research HCG products. Use the full supplied volume (typically 2–5 mL per 5000 IU vial) and refrigerate after mixing.
Do I need a prescription to buy BAC Water?
BAC Water is classified differently across jurisdictions.Customers are responsible for compliance with their own local regulations.
What happens if I use expired BAC Water?
The risk profile is twofold: (1) the benzyl alcohol preservative may have degraded, reducing antimicrobial protection, and (2) the water itself may no longer be guaranteed pyrogen-free. For any research where data integrity matters, always use in-date BAC Water.
Does BAC Water need refrigeration?
No, unopened vials are stable at controlled room temperature (15–25 °C). After first puncture, many labs still refrigerate opened vials as a belt-and-braces measure even though the 0.9% benzyl alcohol is designed for room-temperature in-use storage.
Can I shake the peptide vial to speed up dissolution?
No. Shaking generates air-water interface turbulence that can fragment peptide structures. Add BAC Water gently down the side of the vial and swirl (do not shake) until dissolved. Most peptides clear within 30–60 seconds.
Is BAC Water the same as saline?
No. Saline is sterile water with added sodium chloride. BAC Water contains only sterile water plus 0.9% benzyl alcohol (no salt). Some peptides are supplied with specific diluent recommendations — check the product-specific documentation before substituting.
How many 10 mL vials do I need for a typical peptide protocol?
Depends on your protocol. A common rule of thumb: one 10 mL BAC Water vial reconstitutes approximately 25 mg of peptide across standard working dilutions. For a 10-vial peptide kit at 5 mg each (50 mg total), order two or three BAC Water vials for headroom.
How much BAC Water should I use to reconstitute a peptide vial?
The volume depends on the peptide dose and desired concentration. A common approach for a 2 mg peptide vial is to add 1 mL of BAC water to achieve a 2,000 mcg/mL solution, making individual dose calculations straightforward. Sterile technique (alcohol-swabbed septum, slow injection down the vial wall to avoid foaming) should be used throughout.
What is the shelf life of a reconstituted peptide vial?
Once reconstituted with bacteriostatic water, most peptides remain stable for 30–60 days stored at 4 °C, attributed to benzyl alcohol’s bacteriostatic properties. Lyophilized peptides stored at −20 °C are stable for 1–2 years. Once reconstituted, avoid freezing the solution — repeated freeze-thaw cycles degrade peptide integrity.
Do you ship BAC Water internationally?
Yes. BAC Water ships worldwide alongside peptide orders via our dedicated peptide courier. Peptide-only orders ship at no extra charge; mixed orders use standard tracked international shipping.
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